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ICMR approves low-cost testing method developed by IIT Kharagpur

ICMR approves low-cost testing method developed by IIT Kharagpur

ICMR approves low-cost testing method developed by IIT Kharagpur
October 22
12:50 2020

The machine cost less than ₹5,000 to make and the test kits would each cost about ₹500, IIT-Kharagpur researchers said.

A new coronavirus diagnostic method, using a low-cost portable unit developed by researchers at the Indian Institute of Technology (IIT), Kharagpur, has been approved by the Indian Council of Medical Research (ICMR) after testing with patient samples showed an accuracy level slightly lower than the gold standard RT-PCR method at a fraction of the cost.

The machine cost under ₹5,000 to make and the test units would each cost about ₹500, IIT-Kharagpur specialists told writers on Wednesday. When the viral RNA was removed, the testing cycle took around 60 minutes. The IIT was in conversation with business makers and new companies, and trusted that the administration would likewise finance the expense of test units, they said.

In equal testing with 200 examples containing a wide scope of viral burdens, IIT-Kharagpur’s COVIRAP technique accurately recognized 108 out of the 115 positive examples distinguished by an RT-PCR machine, and 83 out of 85 negative examples, implying that it has a 94% affectability and 98% particularity in contrast with RT-PCR.

“At first, we were extremely distrustful, yet we were enchanted when it worked. The examines are tantamount [to RT-PCR],” ICMR virologist Mamta Chawla Sarkar told writers on Wednesday.

“This examination holds the ability to recognize very low degrees of viral burdens that some other technique dependent on comparable standards of testing… Practically speaking, this implies that beginning phases of disease can be identified, accordingly secluding the patient and capturing the uncontrolled spread of contamination in the network by means of asymptomatic patients,” she noted.

Just ₹5,000 to make it

Not at all like the RT-PCR machine, which could cost up to ₹25 lakh, and should have been worked by an atomic scholar, the COVIRAP machine cost the IIT scientists just ₹5,000 to make and could be fabricated at even lower cost if economies of scale were applied by business makers, said lead specialist and IIT teacher, Suman Chakraborty.

‘Ideal for use in rustic territories’

“The COVIRAP strategy requires next to no gear and is ideal for use in rustic regions with restricted offices. The versatile unit can even be set on a table in a field, without the requirement for a cooled lab. It very well may be worked by country youth with insignificant preparation,” said Dr. Chakraborty. In any case, the viral RNA would in any case be extricated in a lab.

The machine utilized an isothermal nucleic corrosive intensification technique, which got rid of the requirement for a thermocycler, he said. When the example was handled in the machine in the wake of being blended in with arrangements created by the IIT group, treated paper strips – like the pregnancy strips – are dunked into it, and the rise of hued lines will portray the presence of the infection. A portable application has been created to examine the strips set in a hand-held unit, so as to limit human blunder.

Isothermal nucleic corrosive enhancement utilizes a methodology like one in RT-PCR and has its preferences and inconveniences. Different labs, including those of the CSIR (Council of Scientific and Industrial Research) and the Department of Science and Technology, have planned gadgets dependent on this methodology yet none yet have been cleared by the ICMR and arrived at the phase of business fabricating.

FELUDA test

FELUDA, a pack created by the CSIR-IGIB (Institute of Genomics and Integrative Biology), additionally is a ‘paper strip based test’ yet utilizes a quality altering innovation called CRISPR-cas9. Like COVIRAP, it also claims to convey brings about 60 minutes – when contrasted with 3-4 hours in an RT-PCR- – however, are eventually research center put together tests and depend with respect to gifted faculty to separate the nucleic corrosive RNA from a nasopharyngeal test preceding the real testing. FELUDA, cleared by the Drug Controller General of India and the ICMR, has been authorized to Tata Sons and is relied upon to be financially accessible not long from now.

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